Table 2.
VNTR profile of MU isolates detected in various environmental samples
| Localities | Type of samples | Test ID | MIRU-VNTR allelic profile | MU-genotypes | |||
|---|---|---|---|---|---|---|---|
| MIRU-1 | Locus-6 | ST-1 | Locus-19 | ||||
| Akonolinga Centre | Water body | W6AC | 1 | 1 | 2 | 2 | D |
| Water body | W2AC | 1 | 0 | 2 | 0 | ua | |
| Yeme-Yeme | Water body | W2YE | 1 | 1 | 2 | 1 | W |
| Water body | W5YE | 1 | 1 | 1 | 0 | ua | |
| Water body | W7YE | 1 | 1 | 2 | 2 | D | |
| Biofilm | B6YE | 1 | 1 | 2 | 2 | D | |
| Edjom | Water body | W5ED | 0 | 0 | 4 | 0 | UA |
| Biofilm | B2ED | 1 | 2 | 1 | 2 | E | |
| Nyeck | Biofilm | B1NY | 1 | 1 | 2 | 1 | W |
| Endom | Detritus | D2EN | 1 | 0 | 1 | 2 | ua |
MU M. ulcerans, ua unassigned, 0 no amplification; PCR amplification of VNTR-19 was unsuccessful for W2AC and W5ED; PCR amplification of Locus 6 was unsuccessful for W2AC and D2EN; PCR amplification of MIRU-1 was unsuccessful for W5ED; Locus-6 and Locus-19 were the main determinants for a genotype; Genotype E for B2ED corresponds to M. marinum