Fig. 3.

Intrathecal CGRP antagonist and EZH2 inhibitor administration prevent the pain hypersensitivity and attenuate increased levels of EZH2 and H3K27me3 in the spinal dorsal horn induced by CCI. a shows the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) during the 10-day observation period in rats treated with daily intrathecal injection 1 M CGRP, 5 nM GSK126, 2 M CGRP8-37, or vehicle in 10 L for 9 days, respectively. All values are expressed as the means SEMs (n = 6). ^*p < 0.05 vs. sham groups; ^#p < 0.05 vs. CCI alone groups. b Western blot analyses for EZH2 and H3K27me3 protein levels in the spinal dorsal horn with CCI surgery for 3, 5, 7, and 10 days, respectively. Data were obtained from the spinal dorsal horn of animals treated with daily intrathecal injection 1 M CGRP, 5 nM GSK126, 2 M CGRP8-37, or vehicle in 10 L for 2, 4, 6, and 9 days, respectively. The mean optic densities of the proteins were calculated by normalizing to GAPDH. All values are expressed as the means SEMs (n = 4).^*p < 0.05 vs. sham groups; ^#p < 0.05 vs. CCI alone groups.