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. 2021 May 21;19:150. doi: 10.1186/s12951-021-00894-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of BMSCs-derived exosomes. A The morphology of the exosomes and PGZ-Exos was visualized with TEM. Scale bar: 50 nm. B The exosome marker proteins CD9 and CD63 in the exosomes and PGZ-Exos were detected with western blotting. C The size distribution of exosomes and PGZ-Exos was examined with NTA. D PKH26-labeled exosomes and PGZ-Exos uptake by HUVECs was investigated with laser scanning confocal microscopy. The cytoskeleton, exosomes and cell nucleus are stained green, red, and blue respectively. Scale bar: 10 m