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. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: Mol Cancer Res. 2019 Nov 1;18(2):229–239. doi: 10.1158/1541-7786.MCR-19-0688

Figure 6.

Figure 6.

Identifying a novel mediator for MSLN activity using RNAseq and qPCR. A, Analysis schema for RNAseq transcriptional profiling. Thirteen genes were identified to be differentially expressed with at least a 1.5-fold change in expression and P < 0.05 in both KO#1 and KO#2 cell lines and tumor tissues as compared with Mock. B, qPCR showing PPP1R1B relative gene expression in KLM1 cell lines ***, P < 0.001, N.S. = not significant (Student t test with Welch correction). C, Immunoblot showing PPP1R1B expression in KLM1-cultured cell lysates. GAPDH was used as loading control.