Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 7;12:679582. doi: 10.3389/fendo.2021.679582

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2021 Yang, Guo, Peng, Chen, Zhou, Wang, OuYang, Yu and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Single-Cell Transcriptome Profiling of Trophoblast Cells. (A) t-SNE plot grouping 10,236 trophoblast cells into three subtypes: VCT, EVT and SCT. (B) Heatmap showing expression levels for the top five markers for distinguishing VCT, EVT and SCT. (C) Three novel markers were identified by immunofluorescence analysis. SLC1A6, ADRB1, SLC1A2 can be used to distinguish EVT, VCT and SCT respectively. (D) VCT, EVT and SCT were re-clustered into nine, five and three subtypes, respectively. Violin plots show the expression of selected genes within different clusters. (E) Volcano plots of differentially expressed genes (DEGs)in VCT, EVT and SCT. Fifty-eight, 75 and 102 DEGs were identified in VCT, EVT and SCT respectively. Red indicates up-regulated genes and blue indicates down-regulated genes. (F) Differences in pathway activities scored per cell by KEGG analysis between GDM and control group. (G) Pseudotime analysis and RNA velocity of trophoblast cells. The 10,236 trophoblast cells were ordered computationally in terms of RNA velocity (left) and 2D pseudotime trajectory (right).