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. 2021 May 7;15:618393. doi: 10.3389/fncel.2021.618393

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Copyright 2021 Yeates and Frank.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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No discernible NMJ growth defects. NMJs of third instar larvae (same genotypes as Figure 3) were analyzed by immunostaining, co-staining with anti-DLG for the postsynaptic density and anti-Brp to check for apposed presynaptic active zones. (A) DLG boutons counted for Segment A2, NMJ 6/7. (B) DLG boutons counted for Segment A3, NMJ 6/7. No significant differences were found across genotypes (p > 0.9 for every possible head-to-head comparison, one-way ANOVA).