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Ibudilast administration decreased the level of the pyroptosis-related molecule GSDMD in mice with FA-induced AKI. (A) Immunofluorescence labeling of GSDMD, bar = 50 μm. (B) Western blot showing levels of the full-length GSDMD (GSDMD-FL) and active forms of GSDMD (GSDMD-N). (C) Semiquantitative measurements of GSDMD-FL. (D) Semiquantitative measurements of GSDMD-N. Data are shown as the mean ± SEM, n = 5. *p < 0.05, **p < 0.01, and ***p < 0.001.
