FIGURE 9.

Ibudilast administration downregulated the NF-κB signaling pathway in mice with FA-induced AKI. (A) Immunofluorescence staining showing NF-κB p65 nuclear translocation (red arrowheads), bar = 50 μm. (B) Western blot showing p-p65 and p65 levels. (C) Semiquantitative measurements of the ratio of p-p65 to p65. (D) Western blot showing p-IκBα and IκBα levels. (E) Semiquantitative measurements of the ratio of p-IκBα to IκBα. Data are presented as the mean ± SEM, n = 5. *p < 0.05, **p < 0.01, and ***p < 0.001.