HMVEC were pre-treated with ERK1/2 inhibitor (SCH772984; 1μM), or vehicle for 1 hour, and then with LPS (10 ng/ml) for another 6 hours in the continued presence of ERK1/2 inhibitor or vehicle. Levels of (A) IL-6, (B) VCAM-1, (C) IL-8, and (D) PAI-1 were quantified in culture supernatants. n = 4; *p≤0.05; NS, not significant.