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. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: Shock. 2021 Mar 1;55(3):349–356. doi: 10.1097/SHK.0000000000001639

Figure 2: siRNA knockdown of ERK1/2 in HMVEC augments LPS-induced IL-6 production.

Figure 2:

HMVEC were transfected with siRNAs for ERK1, ERK2, or both ERK1 and ERK2 for 24 hours, and then treated with LPS (1 μg/ml). (A) Expression of total ERK1/2 after siRNA transfection by western blotting. Relative density of ERK1/2 was compared to that of β-actin. (B) IL-6 levels in supernatants of LPS-treated HMVEC that had undergone siRNA knockdown of ERK1, ERK2, or both ERK1 and ERK2. n = 4; *p≤0.05; NS, not significant.