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. 2021 May 21;7(21):eabe6903. doi: 10.1126/sciadv.abe6903

Fig. 2. Myeloid cell–specific MYDGF deficiency is associated with atherosclerotic plaque formation in AKO mice.

Fig. 2

AKO and DKO mice aged 4 to 6 weeks were fed a WD for 12 weeks (10 mice in each group). (A and B) The vasodilatation reaction induced by Ach (A) and SNP (B) (n = 10). (C) Representative images of en face atherosclerotic lesions. (D) Quantitative analysis of (C) (n = 5). (E) Representative images of the cross-sectional area of the aortic root (n = 8). Scale bars, 500 μm. (F) Quantitative analysis of (E). (G) Representative immunohistochemical staining images of VSMCs [α–smooth muscle actin (α-SMA)], collagen (Masson), macrophages (anti-CD68), and T lymphocytes (anti-CD3) in aortic plaques. Scale bar, 100 μm. (H) Quantitative analysis of (G) (n = 8). (I and J) The mRNA levels of adhesion molecules (VCAM-1, ICAM-1, and E-selectin) (I) and inflammation (TNF-α, IL-1β, and IL-6) (J) in MAECs of mice (n = 5). The data are presented as the means ± SEM. *P < 0.05 and **P < 0.001.