Fig. 3. Structures of AVP-V2R-G_s-Nb35 complexes in L and T conformations.

(A) Orthogonal views of the cryo-EM density maps of the L state of the AVP-V2R-G_s-Nb35 complex and (B) corresponding model as ribbon representation. V2R is colored in pink, Gα_s in dark blue, Gβ₁ in turquoise, Gγ₂ in yellow, Nb35 in gray, and AVP in cyan. In (B), the distances between W284^6.48 (at its Cα carbon) and the AVP center of mass (COM) and between W284^6.48 and the C-terminal end of α₅ helix of G_s (at the Cα carbon of the free carboxylic acid) are shown. (C and D) Corresponding maps and model for the T state. V2R is colored in blue gray, Gα_s in raspberry, Gβ₁ in turquoise, Gγ₂ in yellow, Nb35 in gray, and AVP in orange. In (D), distances are measured as in (B). (E) L and T models are aligned on the V2R chains, and rotations/translations are shown by measuring displacement (in angstroms) of Gα_s, Gβ₁, Gγ₂, and Nb35.