Skip to main content
. 2021 May 21;7(21):eabf5606. doi: 10.1126/sciadv.abf5606

Fig. 1. Tcf4 is expressed in hippocampal adult neurogenic cells.

Fig. 1

(A) Tcf4 protein expression in the SGZ in adult brain DG. DAPI, 4′,6-diamidino-2-phenylindole. (B) Description for cell type identification used in (C) based on stage-specific marker colocalization and morphology [colored asterisks depict distinct cell types shown on the TCF4 image in (C)]. GCL, Granule Cell Layer. (C) Representative immunofluorescence images showing Tcf4 expression in cells at various stages of adult hippocampal neurogenesis, showing RGL in the top and the other cell types in the bottom; dotted curved lines mark the SGZ area. (D) Tcf4 protein quantitation in different cell stages from (B) and (C) normalized to mature granule cells. Quantitation from three brains; error bar represents SD (unpaired t test; *P < 0.03). Scale bars, 25 μm. MFI, mean fluorescence intensity.