Table 3.
Differences in analyzed parameters between cooperatives.
| Parameter | COOP 1 | COOP 2 | Significance level (P) |
|---|---|---|---|
| Intact membrane (IM) spermatozoa (PI-) (%) | 50.68 ± 1.50 | 41.41 ± 1.84 | P < 0.001 |
| IM non-capacitated (IM-NC) spermatozoa (%) | 19.36 ± 0.74 | 18.45 ± 1.01 | n.s. |
| Non-permeant (NP) sperm with high ΔΨm (YO-PRO-1-/MitoT+) (%) | 26.08 ± 1.57 | 31.65± 2.95 | n.s |
| IM sperm without PS translocation (Annx-/PI-) (%) | 37.92 ± 1.06 | 33.47 ± 1.70 | P = 0.024 |
| IM sperm without active caspases (FITC-VAD-/Eth-) (%) | 33.81 ± 1.29 | 30.97 ± 1.79 | n.s. |
| DNA-intact spermatozoa (TUNEL-) (%) | 83.63 ± 0.53 | 88.21 ± 0.66 | P < 0.001 |
| Oxygen consumption (fmol O2·mL−1·min−1 per million cells) |
0.59 ± 0.14 | 0.58 ± 0.22 | n.s. |
| Fertility (ewes lambing per ewes inseminated, %) | 51.37 ± 1.28 | 53.60 ± 2.12 | n.s. |
| Fecundity (number of lambs born per ewes inseminated) | 0.82 ± 0.02 | 0.84 ± 0.03 | n.s. |
| Prolificacy (number of lambs born per ewe lambing) | 1.56 ± 0.02 | 1.56 ± 0.03 | n.s. |
Results are expressed as a mean value ± standard error of the mean. Last column reflects the level of significance. n.s., non significant.