Table 4.
Differences in analyzed parameters between seminal doses from the breeding and the non-breeding season for both cooperatives.
| Breeding season | Non-Breeding season | |||
|---|---|---|---|---|
| Parameter | COOP 1 | COOP 2 | COOP 1 | COOP 2 |
| Intact membrane (IM) spermatozoa (PI-) (%) | 56.71 ± 2.05 | 43.68 ± 2.40*** | 44.40 ± 2.02 | 38.06 ± 2.82 |
| IM non-capacitated (IM-NC) spermatozoa (%) | 20.23 ± 1.08 | 19.15 ± 1.01 | 18.23 ± 1.00 | 17.36 ± 2.07 |
| Non-permeant (NP) sperm with high ΔΨm (YO-PRO-1 -/MitoT+) (%) |
37.90 ± 2.34 | 34.61 ± 2.69 | 16.82 ± 1.11 | n.d. |
| IM sperm without PS translocation (Annx-/PI-) (%) | 40.72 ± 1.34 | 35.05 ± 2.19* | 34.68 ± 1.62 | 31.16 ± 2.68 |
| IM sperm without active caspases (FITC-VAD-/Eth-) (%) | 33.23 ± 1.55 | 30.39 ± 1.81 | 32.45 ± 1.19 | 33.79 ± 2.05 |
| DNA-intact spermatozoa (TUNEL-) (%) | 84.06 ± 0.73 | 87.87 ± 0.90*** | 83.11 ± 0.79 | 88.72 ± 0.96*** |
| Oxygen consumption (fmol O2·mL−1·min−1 per million cells) |
0.56 ± 0.02 | 0.55 ± 0.02 | 0.61 ± 0.18 | 0.62 ± 0.42 |
| Fertility (ewes lambing per ewes inseminated, %) | 52.19 ± 1.72 | 54.99 ± 2.53 | 50.15 ± 1.96 | 51.46 ± 3.76 |
| Fecundity (number of lambs born per ewes inseminated) | 0.85 ± 0.03 | 0.88 ± 0.05 | 0.79 ± 0.03 | 0.79 ± 0.06 |
| Prolificacy (number of lambs born per ewe lambing) | 1.60 ± 0.03 | 1.57 ± 0.04 | 1.53 ± 0.03 | 1.55 ± 0.06 |
Results are expressed as a mean value ± standard error of the mean.
*
P < 0.05 and
***
P < 0.001 between cooperatives within season. n.d, not enough data to include in the comparative analysis.