Figure 5.
Physiological effects of silencing MC4R PVH neurons. A) In situ hybridization in WT mouse for CalcR (Blue) and Mc4R RNAs (red). Percent expression of CalcR in MC4R positive cells and percent expression of MC4R in CalcR positive cells. B) Bilateral expression of AAV-TetTox-GFP in the PVH of MC4RCre mice (MC4R-Cre + TT). Effect of MC4RPVH silencing on body weight (BW) (C), body weight gain (D), fat and lean mass (E). Food intake in MC4RPVH silenced mice during week 3 (F) and during week 7 (G) following injection. Average values ± SEM are shown for BW, change in BW, body composition, and food intake. P values for BW and change in BW were determined using a 2-way ANOVA followed by Tukey’s multiple comparisons test, and for body composition and food intake, a 1-way ANOVA followed by a Tukey’s multiple comparisons test was conducted. * P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.