FIG 1.

In vitro growth kinetics of ANDV strains Chile-971789 and CHI-7913. Nearly confluent baby hamster kidney (BHK) cells were infected in 24-well dishes with either ANDV Chile-9717869 or CHI-7913 at an MOI of 0.001 (100 μl total volume). Inoculum was removed after 1 h, and cells were washed and cultured for 3, 5, or 7 days postinfection, at which point supernatants were collected and analyzed for the presence of ANDV-RNA by real-time RT-qPCR methodologies. Data shown represent average genomic copies per ml with standard errors of the means (SEM).