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. 2021 Apr 26;95(10):e00140-21. doi: 10.1128/JVI.00140-21

FIG 8.

FIG 8

The rOC/SR903A mutant virus is avirulent in C57BL/6 mice. Twenty-two-day-old C57BL/6 mice received 101.5 TCID50/10 μl of rOC/ATCC, rOC/SG758R, rOC/SR903A, or PBS by the i.c. route. (A) Survival curves of mice over a 21-day period. Differences between the three viruses were significant compared to sham control and to each other (*, P ≤ 0.05, **, P ≤ 0.01, and ***, P ≤ 0.001). (B) C57BL/6 mice were weighed every 2 days over a 21-day period to estimate weight variations, which were expressed as percentages, compared to day 0 (100%). Differences were significant between 7 and 17 dpi, when the three conditions (rOC/ATCC, rOC/SG758R, and rOC/SGR903A) were compared to sham control. (C) Evaluation of the clinical scores (percentage of mice at each level of the scale) of mice infected by rOC/ATCC, rOC/SG758R, or rOC/SGR903A based on neurological symptoms described in a clinical score scale between level 0 and 4 (see Materials and Methods).