UBE1L2 is localized to the cis-Golgi during KSHV lytic replication. (A) In order to induce lytic replication, iVero-BAC16 cells were treated with doxycycline (+dox) or without doxycycline [dox(-)] for 48 h. The cells were then fixed, permeabilized, and stained with anti-GM130 antibody (red), anti-UBE1L2 antibody (yellow), and DAPI (blue). Merged images are shown on the right. (B) Lytic-replication-induced-iVero-BAC16 cells were fixed, permeabilized, and stained with anti-UBE1L2 (red) and anti-GM130 (green) antibodies followed by superresolution microscopy. The merged image is shown on the right.