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. 2021 Apr 26;95(10):e02194-20. doi: 10.1128/JVI.02194-20

FIG 5.

FIG 5

Identification of FAT10ylated proteins. (A) The proteomic workflow designed to isolate and identify proteins linked to His-tagged FAT10 or endogenous FAT10. Proteins linked to His-tagged FAT10 (HisTag-FAT10) or endogenous FAT10 were isolated by His tag affinity chromatography (HisTag Affinity) or immunoprecipitation with anti-FAT10 antibody (FAT10 IP), respectively. The isolated proteins were then identified by mass spectrometry (LC-MS/MS) analysis. (B) A Venn diagram of identified KSHV proteins captured by a Ni-NTA affinity column (HisTag FAT10) or immunoprecipitations with anti-FAT10 antibody (FAT10 IP). (C) The 10 KSHV proteins that were captured by both His tag pulldown and immunoprecipitation with anti-FAT10 antibody. The table lists the UniProt IDs, protein names, and quantitative values of FAT10ylated or His tag FAT10ylated KSHV protein expression during lytic or latent replication. The intensity columns (HisTag-FAT10 and FAT10 IP) show the observed intensities based on label-free quantification. The presence of a 0 indicates that the protein was not identified.