TABLE 1.

Library preparation and RNA-Seq technology used for analysis of IAV PR8 infections of MDCK cells

Parameter	Infection 1, Seq R1	Infection 2, Seq R2	Infection 3
			Seq R3	Seq R4
Library prep method	Clontech Pico SMARTer stranded total RNA-Seq kit v2	Clontech Pico SMARTer stranded total RNA-Seq kit v2	Clontech Pico SMARTer stranded total RNA-Seq kit v2	Illumina TruSeq mRNA
Sequencing method	HiSeq 2500 high output, 50-bp PEa (v4 chemistry)	NovaSeq SPrime 2 × 50-bp PE	NovaSeq SPrime 2 × 50-bp PE	NovaSeq S1 2 × 150-bp PE
Approximate depth (million reads/sample)	16.9	26.8	26.8	20.3

^aPE, paired end.