FIG 3.

Clathrin heavy chain colocalizes with UL32-GFP-positive vesicles in HMVECs and fibroblasts. (A) Uninfected or TB40/E-UL32-GFP-infected HMVECs (96 hpi, MOI 4) or fibroblasts (72 hpi, MOI 2) labeled with anti-clathrin heavy chain (red). In uninfected cells, clathrin is distributed in diffuse puncta throughout the cell. There is substantial colocalization of clathrin heavy chain and UL 32-GFP vesicles (arrows, insets) in both HMVECs and fibroblasts. In fibroblasts, clathrin also accumulates in the VAC (lower middle panel). (B) Quantification of clathrin-positive, UL32-GFP positive vesicles. Thirteen hundred vesicles were counted; *, P < 0.05. Scale bars, 20 μm.