FIG 5.

LAMP1, GM130, and LC3B localize to UL32-GFP-positive vesicles in HMVECs. Uninfected or TB40/E-UL32-GFP-infected HMVECs were labeled with anti-LAMP1 (late endosomes-lysosomes) (A), anti-LC3B (autophagosomes) (B), and anti-GM130 (cis-Golgi) (C) antibodies and secondary antibodies conjugated to Alexa Fluor 647 (red) at 96 hpi (MOI 4). Nuclei, blue. In uninfected cells, LAMP1 and LC3B are distributed throughout the cytoplasm; GM130 is localized to the perinuclear region. In infected cells, LAMP1, LC3B, and GM130 all colocalize on UL32-GFP-positive vesicles (arrows). Scale bars, 20 μm.