TABLE 1.

Antibodies used in this study

Antibody	Speciesa	Source	Clone	Dilution	Cellular compartment
CD63	M	DSHB	H5C6	3 μg/mlb	MVB
LBPA	M	Echelon	ML062915-21	1:100	MVB
Clathrin	R	Cell signaling	4796S	1:50	Endocytic vesicles and cell surface
EEA1	R	Cell signaling	3288	1:100	Early endosome
Rab5	M	BD transduction	610725	1:50	Early endosome
ALIX	M	Invitrogen	MA1-83977	1:200	MVB
Rab7	R	Cell signaling	9367	1:100	Late endosome
p230c	M	BD transduction	611280	1:400	Trans-Golgi
Rab6	R	Cell signaling	9625	1:400	Golgi-ER
Rab11	R	Cell signaling	5589	1:100	Recycling endosomes
Rab14	R	Sigma-Aldrich	R0656	1:200	Endosome-Golgi
AP-2	M	Abcam	ab2807	1:100	Endosome
AP-1d	M	Sigma-Aldrich	A4200	1:100	Trans-Golgi network
AP-3	M	DSHB	SA4	5 μg/mle	Trans-Golgi network
LAMP1	R	Abcam	ab62562	1:500	Late endosome-Lysosome
LC3Bf	R	Cell signaling	3868	1:200	Autophagosome
GM130	M	BD transduction	610822	1:100	cis-Golgi
Anti-GFP	M	DSHB	G1-c 2ea	5 μg/mlg
Alexa Fluor 647	NA	Thermo Fisher	1:1000	NA
Alexa Fluor 568	NA	Thermo Fisher	1:1000	NA
IE1/2	M	Gift from Thomas Shenk, Princeton University	3H4	1:1,000	NA
UL44	M	Virusys	10D8	1:2,500	NA
pp28	M	Gift from Thomas Shenk, Princeton University	10B4-29	1:50	NA
GAPDH	M	Abcam	1:15,000	NA

^aR, Rabbit; M, Mouse.

^bOriginal concentration 71 μg/ml.

^{c, d}A generous gift from Samuel Campos, University of Arizona.

^eOriginal concentration 52 μg/ml.

^fFixed with ice-cold 100% methanol.

^gOriginal concentration 239 μg/ml.