FIG 10.

AP-1 inhibition reduces KDM2B-induced lytic viral gene expression. (A) Flowchart of the experiments. SLK cells were first transduced with lenti-GFP or lenti-3×FLAG-KDM2B for 48 h. Cells were split, and 16 h later the same number of cells was treated with DMSO or 50 μM SR11302. After 24 h, cells were infected with KSHV in the presence or absence of SR11302. Virus was removed at 2 hpi. At 72 hpi, cells were harvested for RNA and protein analysis. (B) The gene expression levels of c-Fos, c-Jun, and IL-6 were analyzed by RT-qPCR. (C) Viral gene expression was measured by RT-qPCR at 72 hpi. (D) Immunoblot analysis of viral ORF6 and ORF45 protein expression at 72 hpi. Tubulin was used as a loading control. (E) AP-1 inhibitor reduced lytic gene expression induced by 3×FLAG-F-box–LRR. The experiment was performed as described for panel B. The viral gene expression was analyzed by RT-qPCR at 72 hpi. t tests were performed between DMSO- and SR11302-treated cells, and P < 0.05 (*) was considered statistically significant.