FIG 11.

siRNA knockdown of AP-1 subunits reduces KDM2B-induced lytic KSHV gene expression. (A) Flowchart of the experiments similar to Fig. 10, except that SLK cells were transfected with 50 nM siRNAs prior to KSHV infection. (B) RT-qPCR analysis of gene expression in siRNA-treated cells. (C) Immunoblotting for c-Fos, c-Jun, and viral ORF6 protein at 72 hpi. Tubulin was used as a loading control. (D) Viral gene expression was measured by RT-qPCR at 72 hpi. t tests were performed between siRNA c-Fos or c-Jun and siControl; *, P < 0.05 was considered statistically significant.