FIG 5.

Overexpression of KDM2B reduces RING1B binding on the viral genome, which depends on the C terminus of KDM2B. SLK cells were first transduced with lenti-GFP or lenti-3×FLAG-KDM2B WT or mutants, which was followed by KSHV infection for 72 h. RING1B ChIP assay was performed to test the binding of RING1B to the indicated regions of the RTA promoter, RTA gene body +0.8 kb downstream of the transcription start site, and the promoter (pr) and the gene body (gb) of E (K2) and L (ORF25) genes. Cellular intergenic region Neg and the MYC gene promoter were used as controls. t tests were performed between GFP-OE and KDM2B-OE samples, and P < 0.05 (*) was considered statistically significant.