FIG 6.

RTA expression in KDM2B-OE cells is responsible for the reduced RING1B binding on the KSHV genome. SLK cells were transduced with lenti-GFP or lenti-3×FLAG-KDM2B for 3 days, followed by infection with WT or RTA-KO KSHV for 3 days. (A) Immunoblot analysis of 3×FLAG-KDM2B expression using anti-FLAG antibody. (B) qPCR measurement of the viral DNA level in KDM2B-OE cells relative to GFP-OE cells at 72 hpi. (C) FLAG ChIP for testing the binding of 3×FLAG-KDM2B on RTA promoter. (D) RING1B ChIP assay for analyzing RING1B-binding on the promoter (pr) and the gene body (gb) of lytic viral genes. (E) Immunoblot analysis of PRC1 factors at 72 hpi. t tests were performed between GFP-OE and KDM2B-OE samples, and P < 0.05 (*) was considered statistically significant; ns, nonsignificant.