FIG 8.

Interaction of KDM2B with SKP1 is necessary for KDM2B-induced de novo lytic KSHV infection. (A) Schematic representation of the full-length KDM2B (WT) and its C-terminal fragments. (B) HEK293T cells were transfected with F-box–LRR and LRR domain expression constructs for 48 h followed by FLAG IPs, which were tested for the presence of endogenous SKP1. (C) SLK cells were transduced with lenti-GFP or lenti-3×FLAG-KDM2B (WT), 3×FLAG-F-box–LRR, or 3×FLAG-LRR for 3 days and then infected with KSHV for 3 days. At 72 hpi, viral gene expression was measured by RT-qPCR and calculated relative to viral gene expression in GFP-OE cells. (D) Immunoblot detection of viral proteins at 72 hpi. Tubulin was used as a loading control. t tests were performed between GFP-OE and KDM2B-OE samples, and P < 0.05 (*) was considered statistically significant.