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. 2021 May 10;95(11):e02453-20. doi: 10.1128/JVI.02453-20

TABLE 2.

Primers used to produce the specific sgRNA for pBAC-PEDV-YN200 digestion

Primer Sequence
sgS-F TTAATACGACTCACTATAGGTAAGACATAGTCATAATTGGTTTTAGAGCTAGA
sgS-R TTAATACGACTCACTATAGGCCATGTTCTTTTTCAGAGCGTTTTAGAGCTAGA
sgRNA AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC