FIG 4.

Inhibitory effect of ilaprazole on HSV-2 and HSV-1 production. Vero cells were infected with HSV-2 or HSV-1 at an MOI of 0.1 and examined by transmission electron microscopy 24 h later. (A to D) HSV-2-infected untreated cells (A and B) and cells treated with 18 μM ilaprazole (C and D). (E to H) HSV-1-infected untreated cells (E and F) and cells treated with 18 μM ilaprazole (G and H). Eighty cells where virus particles were observed were examined. In the presence of ilaprazole, we observe no or very few particles in the cytoplasm (Cyt). Treated cells also have an accumulation of electron-dense material and particles associated with them. In untreated HSV-2-infected cells, we observed an average of 120 particles in the cytoplasm, ranging from 28 to 204, and an average of 16 particles, ranging from 10 to 22, in the nucleus. In treated HSV-2-infected cells, we observed 0 particles in the cytoplasm and an average of 31 particles, ranging from 9 to 56 particles, in the nucleus. In untreated HSV-1-infected cells, we observed an average of 135 particles, ranging from 12 to 152 particles, in the cytoplasm and an average of 35 particles, ranging from 10 to 66 particles, in the nucleus. In treated HSV-1-infected cells, we observed 0 particles in the cytoplasm and an average of 43 particles, ranging from 15 to 166 particles, in the nucleus. Arrows point to virus particles.