FIG 2.

Correlation between HIV-1 vpu/env mRNA level and Env protein level. (A) Sorting strategy for Env-GFP^Bright, Env-GFP^dim, and Env-GFP⁻ cells. Activated CD4⁺ T cells were infected with NL4-3ΔEnv-EGFP. Env-GFP^Bright, Env-GFP^dim, and Env-GFP⁻ cells were sorted based on green fluorescent protein (GFP) intensity on day 3 postinfection. (B) Sorting strategy for IgG-Fc^Bright, IgG-Fc^dim, and GFP⁻ cells. Activated CD4⁺ T cells were infected with NL4-3-Env(Ba-L)-Δnef-EGFP. On day 3 postinfection, surface Env protein was labeled by PGT121 followed with anti-human IgG Fc antibodies. (C and D) Cell-associated vpu/env or gag mRNA was measured by qPCR. P values were calculated by one-way analysis of variance (ANOVA) with Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.