TABLE 1.
List of probes and primers for vpu/env RT-qPCR
| Probe or primer purpose | Probe or primer type | Sequence (5′–3′)a |
|---|---|---|
| gag qPCR | Probe | VIC-CTATCCCATTCTGCAGCTTCCTCATTGATG-TAMRA |
| Forward primer | ACATCAAGCAGCCATGCAAAT | |
| Reverse primer | TCTGGCCT GGTGCAATAGG | |
| tat/rev qPCR | Probe | VIC-TTCCTTCGGGCCTGTCGGGTCCC-TAMRA |
| Forward primer | CTTAGGCATCTCCTATGGCAGGAA | |
| Reverse primer | GGATCTGTCTCTGTCTCTCTCTCCACC | |
| vpu/env qPCR | Probe | FAM- CGCACRGCAAGAGGCGAGGG-MGB |
| Forward primer | AGCTCTCTCGACGCAGGACTC | |
| R1 reverse primer | TACTACTYACTGCTTTGRTAGA | |
| R2 reverse primer | CATYACATGTACTACTYACTGCTTTG | |
| R3 reverse primer | GCATYACATGTACTACTYACTGCTTT | |
| R4 reverse primer | AAAGGTTGCATTACATGTACTACTTACTGCTTT | |
| R5 reverse primer | GCTACTACTAATGCTACTATTGCTAATAT | |
| R6 reverse primer | GCTAATATTTGTAAAGGTTGCATTACATGTACTAC |
a
TAMRA, 6-carboxytetramethylrhodamine; FAM, 6-carboxyfluorescein. The underlined probe and primer were chosen for the vpu/env assay.