TABLE 2.

qPCR conditions

qPCR component or condition	Concn		Cycle length	No. of cycles
	Stock	Final
TaqMan Universal PCR master mix	2×	1×
Forward primer	10 μM	500 nM
Reverse primer	10 μM	500 nM
Probe	2.5 μM	125 nM
Cycle temperature
95°C	2 min
95°C	2 s	40
60°C	20 s	40