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. 2021 May 10;95(11):e00453-21. doi: 10.1128/JVI.00453-21

FIG 1.

FIG 1

Thiopurine analogs 6-TG and 6-TGo selectively induce stress granules in IAV-infected cells. (A) Structures of 6-TG and 6-TGo compared to structurally similar nucleobases and nucleosides. Sulfur atoms in 6-TG, 6-TGo, and 6-MP are highlighted in green. (B and C, top) Circular spot intensity quantification of EGFP-G3BP focus formation in cells infected with IAV-Udorn (blue) or mock-infected cells (red) treated with increasing doses of 6-TG (B) and 6-TGo (C). (Bottom) Representative Cellomics images of the EGFP channel of cells treated with 30 μM 6-TG (B) and 6-TGo (C). (D) Immunofluorescence staining of A549 cells infected with IAV-CA/07 and treated with 6-TG or mock treated for 4 h. The stress granule markers G3BP1 (red) and PABP (green) colocalize in cytoplasmic stress granules (separate channels are shown in the outset). Infected cells are stained with anti-IAV antibody (blue). (E) Immunofluorescence staining of A549 cells treated as described above for panel D for the stress granule markers G3BP1 (red), TIAR (green) (top), and eIF3A (green) (bottom). White lines correspond to pixel intensity profiles shown on the right, as captured using Zeiss ZEN software.