TABLE 1.
Primer sequences for RT-qPCR analysisa
| RT-qPCR target and primer direction | Primer sequence (5′–3′) |
|---|---|
| 5S rRNA | |
| F | GCCCGATCTCGTCTGATCT |
| R | AGCCTACAGCACCCGGTAT |
| BiP | |
| F | GCCTGTATTTCTAGACCTGCC |
| R | TTCATCTTGCCAGCCAGTTG |
| CHOP | |
| F | ATGAACGGCTCAAGCAGGA |
| R | GGGAAAGGTGGGTAGTGTGG |
| EDEM1 | |
| F | TTGACAAAGATTCCACCGTCC |
| R | TGTGAGCAGAAAGGAGGCTTC |
| ERdj4 | |
| F | CGCCAAATCAAGAAGGCCT |
| R | CAGCATCCGGGCTCTTATTTT |
| GAPDH | |
| F | ACGAATTTGGCTACAGCAACAGGG |
| R | TCTACATGGCAACTGTGAGGAGG |
| HERPUD1 | |
| F | AACGGCATGTTTTGCATCTG |
| R | GGGGAAGAAAGGTTCCGAAG |
| PR8 HA | |
| F | CTGGACCTTGCTAAAACCCG |
| R | TCTGGAAAGGGAGACTGCTG |
| PR8 NA | |
| F | TCACTTGGAATGCAGGACCT |
| R | CGATTGTTAGCCAGCCCATG |
a
F, forward; R, reverse; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.