FIG 1.

RSV-neutralizing activity in Pichia pastoris culture supernatants. VHHs were produced in 400-μl P. pastoris cultures in 96-deep-well plates. Ten-fold serial dilutions (from 1/100 to 1/10,000) of the cleared culture supernatants were tested for RSV A2-neutralizing activity in a 96-well-formatted plaque-reduction assay. Boxes indicate P. pastoris clones that secrete VHHs that may have neutralizing activity. Supernatants of F-VHH-4-expressing P. pastoris cells were included as a positive control.