TABLE 2.

RG fold change and stability parameters of the six tested RGs in CD4⁺ T cells^a

Condition	RG	6h					24h
		Fold change			geNorm M	BestKeeper SD	Fold change			geNorm M	BestKeeper SD
		Mean	Upper 90% CI	Lower 90% CI			Mean	Upper 90% CI	Lower 90% CI
PHA/DMSO	TBP	1.08	1.27	0.93	0.30	0.13	1.55	1.87	1.29	0.55	0.32
	RPL27A	0.98	1.11	0.86	0.34	0.10	1.48	1.90	1.15	0.58	0.28
	UBE2D2	1.22	1.45	1.03	0.30	0.16	2.26	2.76	1.86	0.56	0.59
	HPRT1	2.18	2.41	1.97	0.58	0.56	4.08	5.37	3.09	0.64	1.01
	YWHAZ	0.84	1.08	0.66	0.42	0.18	2.06	2.27	1.87	0.53	0.52
	GAPDH	1.71	2.09	1.40	0.41	0.42	7.57	10.73	5.35	1.01	1.46
PMAi/DMSO	TBP	1.39	1.55	1.25	0.83	0.25	2.96	3.38	2.59	0.64	0.78
	RPL27A	1.06	1.06	1.05	0.81	0.14	1.86	2.03	1.70	0.81	0.45
	UBE2D2	0.77	0.90	0.66	0.91	0.23	4.16	4.92	3.51	0.69	1.03
	HPRT1	5.31	6.35	4.44	1.58	1.20	7.35	10.98	4.92	0.83	1.44
	YWHAZ	0.10	0.11	0.08	2.17	1.68	1.32	1.75	1.00	0.99	0.23
	GAPDH	1.65	2.01	1.36	0.92	0.43	12.18	16.91	8.76	1.12	1.80
VOR/DMSO	TBP	1.00	1.13	0.90	0.17	0.18	0.92	1.02	0.84	0.26	0.07
	RPL27A	1.01	1.14	0.89	0.23	0.13	1.05	1.20	0.91	0.20	0.10
	UBE2D2	1.24	1.33	1.16	0.21	0.25	1.19	1.40	1.01	0.32	0.17
	HPRT1	1.09	1.22	0.98	0.25	0.31	1.14	1.20	1.09	0.22	0.18
	YWHAZ	1.00	1.17	0.85	0.20	0.25	1.02	1.06	0.97	0.21	0.12
	GAPDH	1.25	1.44	1.08	0.22	0.33	1.58	1.77	1.41	0.37	0.35
ROM/DMSO	TBP	0.70	0.79	0.63	0.30	0.26	1.27	1.54	1.05	0.46	0.21
	RPL27A	0.97	1.06	0.88	0.24	0.17	0.99	1.11	0.89	0.46	0.08
	UBE2D2	0.99	1.12	0.87	0.23	0.20	0.92	1.02	0.84	0.55	0.16
	HPRT1	0.78	0.87	0.69	0.36	0.34	1.25	1.42	1.10	0.48	0.24
	YWHAZ	0.98	1.06	0.90	0.22	0.23	0.32	0.34	0.29	1.11	0.83
	GAPDH	1.01	1.14	0.89	0.27	0.31	2.49	2.97	2.10	0.86	0.66
PEP005/DMSO	TBP	1.01	1.06	0.95	0.43	0.16	1.06	1.15	0.98	0.45	0.07
	RPL27A	0.89	1.07	0.74	0.51	0.17	1.06	1.18	0.95	0.45	0.06
	UBE2D2	1.47	1.79	1.21	0.38	0.28	1.70	2.12	1.37	0.44	0.38
	HPRT1	2.01	2.48	1.62	0.49	0.50	1.50	1.75	1.29	0.38	0.30
	YWHAZ	1.17	1.34	1.02	0.39	0.26	1.53	1.89	1.24	0.38	0.33
	GAPDH	3.05	3.71	2.51	0.74	0.81	3.61	4.69	2.78	0.81	0.93
AZD/DMSO	TBP	0.92	1.07	0.79	0.17	0.17	0.97	1.07	0.87	0.17	0.06
	RPL27A	0.96	1.10	0.84	0.20	0.16	0.98	1.11	0.86	0.16	0.10
	UBE2D2	0.92	1.21	0.70	0.19	0.24	1.00	1.12	0.89	0.30	0.12
	HPRT1	0.82	0.94	0.71	0.31	0.24	1.00	1.26	0.79	0.20	0.22
	YWHAZ	0.89	1.01	0.79	0.18	0.24	0.99	1.08	0.90	0.16	0.13
	GAPDH	0.98	1.12	0.86	0.24	0.30	1.04	1.18	0.92	0.17	0.17
IBET/DMSO	TBP	0.89	1.01	0.79	0.30	0.14	0.85	1.04	0.70	0.19	0.16
	RPL27A	0.92	1.17	0.73	0.29	0.22	0.89	1.01	0.79	0.19	0.12
	UBE2D2	0.95	1.19	0.76	0.25	0.25	1.00	1.20	0.84	0.32	0.15
	HPRT1	0.93	1.20	0.71	0.28	0.29	0.84	1.00	0.71	0.24	0.22
	YWHAZ	0.50	0.63	0.39	0.54	0.55	0.70	0.79	0.62	0.28	0.26
	GAPDH	1.11	1.21	1.02	0.34	0.30	1.14	1.23	1.06	0.30	0.16
STINGa/DMSO	TBP	0.85	0.87	0.82	0.33	0.18	0.81	0.95	0.70	0.25	0.15
	RPL27A	0.81	0.96	0.68	0.33	0.22	0.67	0.80	0.56	0.19	0.29
	UBE2D2	0.65	0.70	0.59	0.28	0.33	0.77	0.85	0.71	0.31	0.24
	HPRT1	0.75	0.86	0.65	0.32	0.31	0.65	0.77	0.54	0.20	0.31
	YWHAZ	0.39	0.44	0.35	0.54	0.68	0.61	0.67	0.55	0.22	0.36
	GAPDH	0.63	0.73	0.55	0.33	0.41	0.56	0.74	0.42	0.29	0.42

^aRelated to Fig. 2B. RG fold change expressed as average and 90% CI upper and lower limits under LRA conditions relative to that in DMSO in CD4⁺ T cells (n = 4) after 6 h and 24 h. M stability value of geNorm and SD value of BestKeeper in data set composed of two conditions (DMSO plus one LRA) from CD4⁺ T cells after 6 h or after 24 h. Fold change of >2 or <0.5, M value of >1.5, and SD of >1 indicate unstable RGs (highlighted in boldface font).