FIG 10.

LAT expression decreased when Rad21 enrichment at the CTRL2 insulator decreased. (A and B) HeLa cells (A) and differentiated LUHMES cells (B) were infected with wt or ΔCTRL2 virus at 0.1 and 1.0 MOI in triplicate. After 6 h, cells were harvested and RNA was extracted. qRT-PCR was performed for LAT intron primer and probe (Table 1). Threshold values used for PCR analyses were set within the linear range of PCR target amplification based on a standard curve generated for each plate. Viral transcripts were normalized to GAPDH. Statistical analyses were performed with a Student's t test (***, P < 0.0001).