FIG 8.

Deletion of the CTRL2 insulator in HSV-1 decreased Rad21 enrichment 7 and 14 dpi. ChIP assays using the Rad21 antibody or the IgG control were done on mouse TG infected with the ΔCTRL2 virus at 7 and 14 dpi (n = 4 to 5). Subsequent qPCR using primers and custom probes listed in Table 1 were done on both the antibody aliquot and the IgG control. Relative copy numbers in the B, I, or IgG fractions were determined from the equation for the standard curve specific to the primer/probe set and then normalized to the cellular control Tsix Site A B/I for that ChIP assay for comparison across time points. Bar graphs are presented for B/I and IgG/I, and one-way ANOVA on correlated samples was used to determine statistical significance for the enrichment of Rad21 relative to IgG for the individual dpi (*, P < 0.05). (A) Normalized B/I and IgG/I for CTRL1 insulator upstream LAT. (B) Normalized B/I and IgG/I for CTRL2 insulator downstream LAT. (C) Normalized B/I and IgG/I for CTam insulator upstream ICP0. (D) Normalized B/I and IgG/I for CTRS1/2 insulator downstream ICP4. (E) Normalized B/I and IgG/I for CTRS3 insulator upstream ICP4.