Figure 5. Activated caspase3 (aCaspase3) is not observed in embryonic V1^R at E14.5.

Representative confocal image of the ventral part of an isolated lumbar spinal cord of E14.5 GAD67-eGFP mouse embryo showing immunostainings using antibodies against eGFP (A), FoxD3 (B), and aCaspase3 (C). (D) Superimposition of the three stainings shows that embryonic V1^R (eGFP+ and FoxD3+) were not aCaspase three immunoreactive. (A1, B1, C1, and D1). Enlarged images from (A), (B), and (C) showing that aCaspase3 staining is localized in areas where eGFP and Foxd3 staining were absent. (A2, B2, C2, and D2) Enlarged images from (A), (B), and (C) showing that aCaspase3 staining is absent in the area where V1^R (eGFP+ and FoxD3+) are located. aCaspase3 staining that did not co-localize with GAD67eGFP likely indicates motoneuron developmental cell death.