Fig. 2.

Influence of growth medium or nutrient (a), amino acid or purine (b), pH (c), conidial age (d) and temperature (e) on the frequencies of active conidia and active conidia involved in CAT-mediated fusion (V. dahliae isolate 123V). Each medium/condition was tested in triplicate, and 300 conidia were analyzed per replicate. Bars = SD. CzD Czapek-Dox medium, SXM simulated xylem sap medium, β-GP β-glycerophosphate, HX hypoxanthine, ino inosine. Statistical significance of differences from the control (in bold characters in each plot) was tested by Student’s t-test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). The results from the remaining amino acids are presented in Fig. S1