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. 2021 May 21;4:613. doi: 10.1038/s42003-021-02126-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Culture medium was collected at defined time points after seeding and imaged with AD-PAINT. a Super-resolution images of extracellular aggregates. Secreted aggregates accumulate over time following seeding (b), while their length (c) and eccentricity (d) increase slightly. Statistical difference was determined by two-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. Mean ± SD from three biological replicates. Scale bars in a: 500 nm (main panels) and 25 nm (insets).