Fig. 2.

The over-expression of the CaGrx gene increase drought and salinity tolerance in Arabidopsis. a The germination efficacy of Arabidopsis seeds of LOC101493651 together with control (Col-0) in ½ MS plate augmented with 300 mM mannitol. (1) Col-0 seeds inoculated in ½ MS medium, (2) Col-0 seeds in ½ MS + 300 mM mannitol, and (3) transgenic seeds inoculated in ½ MS + 300 mM mannitol. b The germination efficacy of Arabidopsis seeds of LOC101493651 with control (Col-0) in ½ MS plate augmented with 200 mM NaCl. (I) Col-0 seeds inoculated in ½ MS medium, (II) Col-0 seeds in ½ MS + 200 mM NaCl, and (III) transgenic seeds inoculated in ½ MS + 200 mM NaCl. Morphological growth of root, as well as the measurement of root length in transgenic Arabidopsis of LOC101493651 in ½ MS plate, augmented with 300 mM mannitol (c, e) and ½ MS plate, augmented with 200 mM NaCl (d, f) after five days. g After ten days of drought by with-holding water and salinity by 200 mM NaCl, transgenic Arabidopsis plants of LOC101493651 showed enhanced growth in comparison to their wild-type (WT) plants. Control (C) plants were maintained in well-watered controlled conditions throughout experiments. The standard error was estimated from the mean of three replicates. The estimated data were applying to one way ANOVA through DMRT (Duncan’s Multiple Range Test) to the estimation of the significant difference among the means (p < 0.05)