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. 2021 Apr 20;27(5):1089–1104. doi: 10.1007/s12298-021-00993-5

Table 1.

List of gene-specific primers used for RT-PCR analysis

Gene name Genbank accession Forward primer (5′-3′) Primer efficiency (%)
OsHMA2 AP004278 F: ACCAGCTGATTACAAACAAGCAT 98.6
R: CAGTCCTTTACTTCCTCGACCTC
OsHMA3 AP005246 F: CTTCTTTACTGGATTGCAAGCAT 98.8
R: GGTCAGCATAACCGACTTGATG
OsHMA4 AP004184 F: TGCTGGGGAAATATCTGGAG 98.4
R: CTTCTGAACTGGAGCCCTTG
ABCC1 NM_001036039 F: TCGAACTGTGGCAGTCTTTG 98.4
R: AGTCCATTCAATGCCTCACC
PCS1 LC192429 F: TCGCTTCAAATACCCTCCTC 98.8
R: TTTACTTGGGCTGGATCCTC
PCS2 LC192431 F: CAGGGGGTTCATGCTTATCT 98.5
R: GGCAGGAAGGGATTTCACTA
Actin XM_015774830 F: TCCTCCGTGGAGAAGAGCTA 98.8
R: GCAATGCCAGGGAACATAGT