Table 2.
Predictive score for death in ventilated COVID-19 patients.
| Stimulated blood: | ||||||
|---|---|---|---|---|---|---|
| 77 complete datasets of ventilated patients | Right positive (of 8 dead) | False positive (of 69 survived) | Sens. | Spe. | NPV | PPV |
| Weak upregulation of CD123 on Monos + weak upregulation of CD11b on Neutros + low Basophil count | 6 | 0 | 75% | 100% | 94% | 100% |
| Weak upregulation of CD11b on Neutros + low Basophil count | 6 | 1 | 75% | 99% | 94% | 93% |
| Weak upregulation of CD123 on Monos + weak upregulation of CD11b on Neutros | 7 | 3 | 88% | 96% | 97% | 83% |
| Weak upregulation of CD123 on Monos + low Baso count | 7 | 6 | 88% | 92% | 97% | 71% |
| Weak upregulaiton of CD11b on Neutros | 7 | 7 | 88% | 90% | 97% | 67% |
| Weak upregulation of CD123 on Monos | 8 | 15 | 100% | 80% | 100% | 52% |
| Low Baso count | 7 | 32 | 88% | 54% | 95% | 31% |
Three parameters were used individually or in combination to predict fatal outcome in ventilated COVID-19 patients. Absolute basophil counts in fresh peripheral blood. Basophil counts <25/µl defined “Low Baso count”. Upregulation of CD123 on CD14 + monocytes and CD11b on neutrophils defined as the ratio of surface marker expression with anti-CD3 to surface marker expression without anti-CD3. Upregulation of <130% defined “Weak upregulation”. In the combined scores, a logical AND combination was used that required all parameters to be fulfilled. For all three parameters, 77 datasets from 21 ventilated COVID-19 patients (n = 77 biologically independent samples) were available. In all, 17 ventilated patients were discharged from the ICU (survived) (n = 69 biologically independent samples) and 4 patients died on the ICU (n = 8 biologically independent samples). The number of right and false positive samples, the test sensitivity (Sens.), specificity (Spe.), negative (NPV), and positive predictive value (PPV) for predicting death are shown.