Fig. 3. In response to increasing concentrations of doxorubicin, GDF5 undergoes gene expression changes and alters HCM cell viability when silenced.

a Expression levels of GDF5 at increasing concentrations of doxorubicin were measured in HCM cells, normalised to the housekeeping gene HPRT1, and compared using one-way ANOVA to baseline (0 nM doxorubicin). Two independent experiments were performed (total n = 6) and their results combined. ****P < 0.0001 after Bonferroni multiple testing correction. Data are presented as mean and standard deviation. b Expression levels of GDF5 were silenced with RNAi and HCM cell viability at increasing concentrations of doxorubicin was measured using an MTT assay (n = 5). Dose-response curves were fitted to a non-linear regression, log(inhibitor) versus normalised response 4 parameter model and data are presented as mean and standard deviation. siNT represents negative control non-targeting siRNA. The logarithm of the half maximal inhibitory concentrations (IC₅₀) are shown.