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. 2021 May 21;12:3028. doi: 10.1038/s41467-021-23187-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Neuronal activity was monitored in frontal cortex of anesthetized mice. Scheme of coronal section, indicating the expression of GCaMP6s in cortex assessed through a cranial window. Magnified view of imaged cortical area demonstrates neuronal expression of GCaMP (green) across all cortical layers. b Timeline of experiments. Male and female mice were injected with AAV9-syn-jGCaMP7s (at 3 months of age) or AAV2/1-hsyn-GCaMP6m (at 9 months of age) into frontal cortex and implanted with a cranial window. In vivo imaging began 4 weeks after implantation. c Representative examples (average projections) of field of views (FOV) imaged in Fus^+/+ and Fus^ΔNLS/+ mice at 4 months (N = 8 Fus^+/+ mice and N = 3 Fus^ΔNLS/+ mice, left) and at 10 months (N = 5 Fus^+/+ mice and N = 6 Fus^ΔNLS/+ mice, right) are shown together with fluorescence calcium traces of selected regions of interest (ROIs). d The fraction of active cells per FOV was not affected in 4-month-old Fus^ΔNLS/+ mice. N = 13 FOVs in 3 Fus^ΔNLS/+ and N = 25 FOVs in 8 Fus^+/+ mice. Data are presented as mean ± SEM p = 0.1627, Two-sided Unpaired Student’s t-test. e, f The calcium transient frequencies (e) were increased while the average transient amplitudes (f) were decreased in Fus^ΔNLS/+ mice. N = 1107 ROIs in 3 Fus^ΔNLS/+ and N = 2264 ROIs in 8 Fus^+/+, superimposed by the median (e). Kolmogorov–Smirnov test, ***p < 0.0001 for both panel e and f. g–i The fraction of active cells per FOV (g) as well as (h) the frequencies and (i) the average amplitudes of calcium transients of each ROI were increased in 10-month-old Fus^ΔNLS/+ mice. Data are individual FOVs (g; N = 14 FOVs in 6 Fus^ΔNLS/+ and N = 10 FOVs in 5 Fus^+/+ mice) or individual ROIs (h, i; N = 855 ROIs in 6 Fus^ΔNLS/+ and N = 631 ROIs in 5 Fus^+/+ mice) superimposed by the mean ± SEM (g) or the median (h, i). panel g: Two-tailed Unpaired Student’s t-test, *p = 0.0126; panel h and i: Kolmogorov–Smirnov test, ***p < 0.0001 for both panels. Source data are provided as a Source Data file.