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. 2021 May 21;12:3016. doi: 10.1038/s41467-021-23299-2

Fig. 5. RNAse H1 overexpression partially phenocopies RTEL1 deficiency.

Fig. 5

a Example chromosomes, and quantitation of TERRA free ends per chromatid in Tel FISH-positive chromatid ends. Samples were processed by sequential TERRA-Tel FISH and scored for TERRA (red) in telomeres marked by Tel FISH signal (green). Chromatid ends in at least 25 metaphases in two experiments were counted (****p < 0.0001, two-sided t-test). TERRA levels are elevated after RNAse H1 overexpression. b TERRA levels of specified chromosome ends were tested in cells transfected with RNAse H1-GFP. Bars represent the relative TERRA expression measured by qRT-PCR in three independent experiments p-values are listed in Supplementary Fig. 10B (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-sided t-test). Means and standard deviation is shown. c Quantification of TERRA loss per chromatid in cells transfected with RNAse H1-GFP by TERRA FISH (22 days in selection). d Quantification of telomere loss per chromatid in cells transfected with RNAse H1-GFP by telomere FISH (22 days in selection). e Quantification of telomere loss per chromatid in cells transfected with RNAse H1-GFP by telomere FISH (52 days in selection). Three independent cell lines complemented with RTEL1 were averaged for analysis where specified. FISH experiments represent the average of at least 50 metaphases in each of 2 independent experiments and three independent reconstitutions shown as the mean and standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-sided t-test) (see also Supplementary Fig. 10).