Table 2.
Human Pluripotent Stem Cell Line-Specific Differentiation Protocols
| Day | hPSC line-specific differentiation protocols |
|
|---|---|---|
| WTC11 | H9 | |
| D-2 | Seed @ ∼75–150k cells/cm2 in mTeSR1 w/5 μM Y27632 | Seed @ ∼225–350k cells/cm2 in mTeSR1 w/5 μM Y27632 |
| D-1 | mTeSR1 | |
| D0 | RPMI-1640 with B27 − insulin (B27−) + 5 μM CHIR99021 | |
| D1 | — | RPMI-1640/B27− |
| D2 | RPMI-1640/B27− with 5.0 μM IWP2 | — |
| D3 | — | 50% RPMI-1640/B27− + 50% conditioned medium + 5.0 μM IWP2 |
| D4 | RPMI-1640/B27− | — |
| D5 | — | RPMI-1640/B27− |
| D6 | RPMI-1640/B27 | — |
| D7 | — | RPMI-1640/B27 |
| D8 | RPMI-1640/B27 | |
| D10 + 3n | RPMI1640/B27 | |